A rapid, easy-to-use DNA amplification method at 37°C: Study could advance molecular diagnosis and robotics

ScienceDaily | Jun 14, 2019 at 1:16 PM
  • Researchers from Tokyo Institute of Technology (Tokyo Tech), Abbott Japan Co., Ltd, and the University of Electro-Communications, Japan, report a way to achieve million-fold DNA amplification and targeted hybridization that works at body temperature (37°C/98.6°F).
  • The method, named L-TEAM (Low-TEmperature AMplification), is the result of more than five years of research and offers several advantages over traditional PCR, the dominant technique used to amplify DNA segments of interest.
  • In their study published in Organic & Biomolecular Chemistry, the researchers introduced synthetic molecules called locked nucleic acids (LNAs) into the DNA strands, as these molecules are known to help achieve greater stability during hybridization.